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KMID : 1141020160160020069
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Journal of Applied Oriental Medicine
2016 Volume.16 No. 2 p.69 ~ p.78
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Inhibitory effect of Diospyroskaki, Chenogdo flat persimmon on cell differentiation and lipid metabolism-related protein in 3T3-L1 preadipocyte (pilot study)
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Kim So-Young
Park Hae-Jin
Choi Soo-Min
An Hee-Duk
Kim Mi-Ryeo
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Abstract
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Objective : In this study, we investigated the preliminary anti-obesity capacity of water extract from Bansi (Diospyros kaki. CBS), seedless persimmon produced in Cheongdoin using 3T3-L1 preadipocyte.
Methods : The cellular toxicity of CBS extract (CBSE) was measured by MTT assay. And antiobesity-related activities were evaluated via Oil-Red-O staining for monitoring inhibitions on adipogenesis and lipid accumulations, and western blot for identifying expression of differentiation factor (AMPK, ACC, PPARγ, C/EBPα) in 3T3-L1 cells.
Results : Lipid accumulation of CBSE-treated group significantly decreased in a dose dependent manner compare to that of vehicle-treated control group, which is 83.7% and 72.7% at 1㎎/㎖and 2㎎/㎖ CBSE concentration, respectively. And pAMPK and pACC protein expression of 2mg/ml CBSE-treated groups were higher than vehicle-treated control group. The expression levels of PPARγ and C/EBPα in 2㎎/㎖ CBSE-treated groups were lower than control group.
Conclusion : Therefore, these results suggest that the CBSE could be used as a new material for functional food and drink to improve overweight or obesity.
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KEYWORD
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Antiobesity, water extract of Cheongdo Bansi, 3T3-L1, pAMPK, PPARγ
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